What is the clinical evidence from this study?

Study design: Observational. Intervention: Long-chain fatty acids (LCFAs). Primary outcome: Correlation of plasma ANGPTL4 with fasting nonesterified fatty acids and adipose tissue lipolysis (p=0.0036).

What question did this study set out to answer?

To identify novel long-chain fatty acid target genes in human skeletal muscle cells.

December 16, 2008DiabetesOpen Access

Muscle-Derived Angiopoietin-Like Protein 4 Is Induced by Fatty Acids via Peroxisome Proliferator–Activated Receptor (PPAR)-δ and Is of Metabolic Relevance in Humans

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Key result

Plasma ANGPTL4 positively correlated with fasting nonesterified fatty acids (P=0.0036) and adipose tissue lipolysis (P=0.0012) in humans, demonstrating its systemic metabolic relevance.

Population

Human skeletal muscle cells, C2C12 cells, and two human cohorts

Comparison

Long-chain fatty acids and pharmacological… vs Control conditions and PPARG knockdown

Design

Preclinical

Authors

HSHarald StaigerHelmholtz Zentrum München CHCarina HaasUniversity Children's Hospital Tübingen JMJürgen MachannGeneral / Preventive / Lipids

Discussion

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Implication

Hypothesis-generating for ANGPTL4 in lipid metabolism; prospective human trials needed before clinical relevance.

Key Points

To identify novel long-chain fatty acid target genes in human skeletal muscle cells.
In vitro culture and treatment of human myotubes and C2C12 cells
Gene array analysis, real-time RT-PCR, Western blotting, and ELISA
Characterization of human subjects through various metabolic tests
ANGPTL4 is a key LCFA-responsive gene in myotubes, with PPAR-delta activation leading to its production (P = 0.0036)
Knockdown of PPARD inhibits ANGPTL4 induction from LCFAs, showing PPAR-delta’s role in gene regulation
In vivo and in vitro ANGPTL4 levels correlate with adipose tissue lipolysis (P = 0.0012)

Study Design

Type

Observational (n=146)

Structured PICO

Population

Human skeletal muscle cells (myotubes), C2C12 cells, and two human cohorts (n=108 thoroughly phenotyped subjects and n=38 myotube donors)

Intervention

Long-chain fatty acids (LCFAs) and pharmacological PPAR-delta activation

Comparator

Control conditions and PPARG knockdown

Outcome

ANGPTL4 gene expression, production, and secretionsurrogate

Main Result

p-value: p=0.0036

Muscle-derived ANGPTL4 is induced by long-chain fatty acids via PPAR-delta and is associated with systemic lipid metabolism and lipolysis in humans.

Cite This Study

Staiger et al. (2008) reported an observational. Long-chain fatty acids (LCFAs) was evaluated on Correlation of plasma ANGPTL4 with fasting nonesterified fatty acids and adipose tissue lipolysis (p=0.0036). Plasma ANGPTL4 positively correlated with fasting nonesterified fatty acids (P=0.0036) and adipose tissue lipolysis (P=0.0012) in humans, demonstrating its systemic metabolic relevance.

synapsesocial.com/papers/6a0ec300aa1655e5fb22c33d https://doi.org/10.2337/db07-1438

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Muscle-Derived Angiopoietin-Like Protein 4 Is Induced by Fatty Acids via Peroxisome Proliferator–Activated Receptor (PPAR)-δ and Is of Metabolic Relevance in Humans

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Main Result

Cite This Study

Muscle-Derived Angiopoietin-Like Protein 4 Is Induced by Fatty Acids via Peroxisome Proliferator–Activated Receptor (PPAR)-δ and Is of Metabolic Relevance in Humans

Discussion

Member takes

Implication

Key Points

Study Design

Structured PICO

Main Result

Cite This Study

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