What question did this study set out to answer?

To identify physiological determinants of plasma ANGPTL4 levels in humans, focusing on the effects of energy restriction, exercise, and circulating free fatty acids.

April 3, 2009Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Caloric Restriction and Exercise Increase Plasma ANGPTL4 Levels in Humans via Elevated Free Fatty Acids

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Why the study?

Do fasting, caloric restriction, and exercise increase plasma ANGPTL4 levels in humans?

Population

Humans and cell lines (rat hepatoma FAO cells, human primary myocytes, mouse intestinal MSIE cells)

Comparison

Long-term fasting, chronic caloric restriction… vs Baseline or control treatment

Design

Other

Authors

SKSander KerstenPreventive Cardiology LLLaeticia LichtensteinUniversity of Leeds ESEmma SteenbergenTinbergen Institute

Discussion

Member takes

Implication

No immediate clinical application; leaves open ANGPTL4 as a metabolic stress biomarker for future research.

Key Points

To identify physiological determinants of plasma ANGPTL4 levels in humans, focusing on the effects of energy restriction, exercise, and circulating free fatty acids.
Developed a quantitative enzyme-linked immunosorbent assay (ELISA) to measure human plasma ANGPTL4 concentrations.
Measured plasma ANGPTL4 levels under diurnal conditions, prolonged fasting, chronic caloric restriction, endurance exercise, intralipid infusion, and beta-adrenergic agonist stimulation.
Tested the effects of fatty acids on ANGPTL4 gene expression in rat hepatoma cells, human primary myocytes, and mouse intestinal epithelial cells.
Baseline plasma ANGPTL4 levels varied widely between individuals and remained stable diurnally, but increased significantly in response to long-term fasting, chronic caloric restriction, and endurance exercise.
Elevation of plasma free fatty acids via intralipid infusion or beta-adrenergic agonist administration significantly elevated circulating ANGPTL4 levels compared with controls.
Exposure to fatty acids markedly induced ANGPTL4 gene expression across human myocytes, rat hepatoma cells, and mouse intestinal cells.

Structured PICO

Do fasting, caloric restriction, and exercise increase plasma ANGPTL4 levels in humans?

Population

Humans and cell lines (rat hepatoma FAO cells, human primary myocytes, mouse intestinal MSIE cells)

Intervention

Long-term fasting, chronic caloric restriction, endurance exercise, intralipid injection, and beta-adrenergic agonist treatment

Comparator

Baseline or control treatment

Outcome

Plasma ANGPTL4 levelssurrogate

Fasting, caloric restriction, and exercise increase plasma ANGPTL4 levels in humans, likely mediated by elevated free fatty acids.

Cite This Study

Kersten et al. (2009) studied this question.

synapsesocial.com/papers/6a7f8efefb3b41a99728bde7 https://doi.org/10.1161/atvbaha.108.182147

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Caloric Restriction and Exercise Increase Plasma ANGPTL4 Levels in Humans via Elevated Free Fatty Acids

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Caloric Restriction and Exercise Increase Plasma ANGPTL4 Levels in Humans via Elevated Free Fatty Acids

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider