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November 30, 2017Journal of Veterinary Internal MedicineOpen Access

All three platelet function tests detected treatment effects of aspirin and clopidogrel in healthy dogs, with Plateletworks using arachidonic acid detecting significant decreases in aggregation (e.g., 80% to 7% with aspirin, p<0.001) in all dogs.

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Why the study?

Do different platelet function tests (Multiplate, PFA-200, Plateletworks) detect the effects of aspirin, clopidogrel, or combination therapy in healthy dogs?

Population

6 healthy laboratory Beagles

Comparison

Aspirin 1 mg/kg, clopidogrel 2 mg/kg, or… vs Placebo

Design

Preclinical, Randomized, Double-blind

Follow-up

1 week per phase

Key result

All three platelet function tests detected treatment effects of aspirin and clopidogrel in healthy dogs, with Plateletworks using arachidonic acid detecting significant decreases in aggregation (e.g., 80% to 7% with aspirin, p<0.001) in all dogs.

Authors

SSSophie SaatiAAAnthony C. G. Abrams‐OggSBShauna L. Blois

Discussion

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Overview

Platelet function tests may detect antiplatelet effects in healthy dogs; leaves open validation for clinical monitoring or diseased patients.

Study Design

Type

RCT (n=6)

Blinding

Double-blind

Randomization

Randomized crossover design

Multicenter

No

Structured PICO

Do different platelet function tests (Multiplate, PFA-200, Plateletworks) detect the effects of aspirin, clopidogrel, or combination therapy in healthy dogs?

P
Population
6 healthy laboratory Beagles, aged 4.8 to 5.4 years, evaluated in a randomized crossover study for 7 days per treatment phase to assess platelet function.
I
Intervention
Aspirin 1 mg/kg, clopidogrel 2 mg/kg, or combination therapy for 1 week each (crossover design)
C
Comparator
Placebo
O
Outcome
Platelet function assessed on days 0 and 7 of each phase using Multiplate Analyzer (MP), Platelet Function Analyzer-200 (PFA), and Plateletworks (PW)surrogate

Main Result

Absolute Event Rate: 7% vs 80%

p-value: p=<0.001

Multiplate, PFA-200, and Plateletworks can detect the antiplatelet effects of aspirin and clopidogrel in healthy dogs, suggesting utility for monitoring therapy.

Limitations

  • Small sample size of only 6 dogs.
  • Study performed only in healthy Beagles, so results may not apply to other breeds or dogs with clinical diseases.
  • Aspirin dosage of 1 mg/kg may have been insufficient to cause maximal inhibition of platelet function.
  • Delays in time to analysis for some Plateletworks samples compared to manufacturer recommendations.
  • Lack of established reference intervals for most of the platelet function tests in dogs.

Cite This Study

Saati et al. (2017) conducted an RCT in Healthy (n=6). Aspirin, clopidogrel, or combination therapy vs. Baseline (Day 0) was evaluated on Platelet aggregation using Plateletworks with arachidonic acid (PW AA) after 7 days of aspirin (p=<0.001). All three platelet function tests detected treatment effects of aspirin and clopidogrel in healthy dogs, with Plateletworks using arachidonic acid detecting significant decreases in aggregation (e.g., 80% to 7% with aspirin, p<0.001) in all dogs.

synapsesocial.com/papers/6a98fffe1c4dac29babd4b99https://doi.org/10.1111/jvim.14886
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Assessment of platelet function in healthy cats in response to commonly prescribed antiplatelet drugs using three point-of-care platelet function tests2016 · 32 citations
  2. 2Platelet aggregometry testing during aspirin or clopidogrel treatment and measurement of clopidogrel metabolite concentrations in dogs with protein-losing nephropathy2020 · 6 citations
  3. 3Platelet function in dogs: breed differences and effect of acetylsalicylic acid administration2007 · 53 citations
  4. 4In vitro and in vivo assessment of platelet function in healthy dogs during administration of a low-dose aspirin regimen2016 · 23 citations
  5. 5Utility of the PFA-100 Instrument and the Novel Multiplate Analyzer for the Assessment of Aspirin and Clopidogrel Effects on Platelet Function in Patients With Cardiovascular Disease2008 · 51 citations