What question did this study set out to answer?

To determine how animal viruses bind to host tissue cells and whether cellular attachment protects viruses from neutralizing antibodies.

May 1, 1935The Journal of Experimental MedicineOpen Access

The Fixation and Protection of Viruses by the Cells of Susceptible Animals

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To determine how animal viruses bind to host tissue cells and whether cellular attachment protects viruses from neutralizing antibodies.
Exposed individual living and killed (heat or ultraviolet light-treated) rabbit tissue cells to suspensions of vaccinia and Shope fibroma virus.
Washed virus-cell complexes repeatedly with Tyrode solution under agitation to evaluate binding stability.
Challenged cell-bound viruses with neutralizing antisera in tissue culture to test for viral inactivation.
Both living and killed cells rapidly and irreversibly bound vaccinia and Shope fibroma viruses, with viruses remaining attached despite repeated washings with Tyrode solution.
Living host cells completely protected attached viruses from neutralizing antisera, whereas viruses bound to killed cells were successfully neutralized.
Shope fibroma virus remained predominantly cell-associated with minimal presence in culture fluid, while vaccinia caused severe cytopathic destruction in rabbit embryo cultures.

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Implication

Experimental study reveals living host cells bind and shield viruses from neutralizing antisera, suggesting a mechanism for viral persistence and therapeutic failure.

Cite This Study

Rous et al. (1935) studied this question.

synapsesocial.com/papers/6a990b381acbc1f77078be04 https://doi.org/10.1084/jem.61.5.657

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Implication

Experimental study reveals living host cells bind and shield viruses from neutralizing antisera, suggesting a mechanism for viral persistence and therapeutic failure.

The Fixation and Protection of Viruses by the Cells of Susceptible Animals

Key Points

Discussion

Implication

Cite This Study

The Fixation and Protection of Viruses by the Cells of Susceptible Animals

Key Points

Discussion

Implication

Cite This Study

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