October 30, 2000The Journal of General PhysiologyOpen Access

Mg2+ Modulates Voltage-Dependent Activation in Ether-à-Go-Go Potassium Channels by Binding between Transmembrane Segments S2 and S3

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Population

Xenopus oocytes expressing mutant Drosophila ether-à-go-go (eag) potassium channels

Comparison

Site-directed mutagenesis of acidic residues in… vs Wild-type eag channels with and without…

Design

Preclinical

Authors

WSWilliam R. SilvermanUniversity of Miami CTChih‐Yung TangNational Taiwan University AMAllan F. MockUniversity of California, Los Angeles

Discussion

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Implication

Pinpoints eag residues mediating Mg2+ effects; extends channel gating mechanisms but leaves clinical translation open.

Structured PICO

Population

Xenopus oocytes expressing mutant Drosophila ether-à-go-go (eag) potassium channels

Intervention

Site-directed mutagenesis of acidic residues in S2 and S3 segments (D278, D327, D284, D319, D274) and exposure to extracellular Mg2+

Comparator

Wild-type eag channels with and without extracellular Mg2+

Outcome

Ionic current kinetics measured using two-electrode voltage clampsurrogate

Extracellular Mg2+ modulates voltage-dependent activation in eag potassium channels by binding to a water-filled pocket between transmembrane segments S2 and S3, specifically coordinating with residues D278 and D327.

Cite This Study

Silverman et al. (2000) studied this question.

synapsesocial.com/papers/6a99bb2ccc1ba4c2647b8ad3 https://doi.org/10.1085/jgp.116.5.663

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Mg2+ Modulates Voltage-Dependent Activation in Ether-à-Go-Go Potassium Channels by Binding between Transmembrane Segments S2 and S3

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mg2+ Modulates Voltage-Dependent Activation in Ether-à-Go-Go Potassium Channels by Binding between Transmembrane Segments S2 and S3

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider