Key result
Feline calicivirus and murine norovirus infectivities were unstable and FCV was vulnerable to low pH (50% inhibitory pH 3.9), whereas coxsackievirus and poliovirus were stable.
Coxsackievirus may be a more appropriate surrogate test virus for disinfectants against human norovirus than Feline calicivirus or murine norovirus due to its robustness.
May support coxsackievirus as norovirus surrogate for disinfectant testing; leaves open human virus validation.
Feline calicivirus (FCV) is frequently used as a surrogate of human norovirus. We investigated eligibility of FCV for anti-viral assay by investigating the stability of infectivity and pH sensitivity in comparison with other viruses. We found that infectivities of FCV and murine norovirus (MNV) are relatively unstable in infected cells compared with those of coxsackievirus (CoV) and poliovirus (PoV) , suggesting that FCV and MNV have vulnerability. Western blotting indicated that inactivation of FCV was not due to viral protein degradation. We also demonstrated sensitivity of FCV to low pH, the 50% inhibitory pH value being ca. 3.9. Since human norovirus is thought to persist longer, in infectivity and to be a resistant virus, CoV, which is robust and not restrained in use as PoV, may be more appropriate as a test virus for disinfectants, rather than FCV and MNV.
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OHMINE et al. (2018) studied Human Norovirus surrogate testing. Feline calicivirus (FCV) and Murine norovirus (MNV) vs. Coxsackievirus (CoV) and Poliovirus (PoV) was evaluated on Stability of infectivity and pH sensitivity. Feline calicivirus and murine norovirus infectivities were unstable and FCV was vulnerable to low pH (50% inhibitory pH 3.9), whereas coxsackievirus and poliovirus were stable.
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