Key result
In rats with unilateral ureteral obstruction, 7-day treatment with losartan or enalapril significantly reduced the circulating lymphocyte apoptotic index from 29.2% to 11.9% and 7.6%, respectively.
Why the study?
Does angiotensin II blockade with losartan or enalapril reduce apoptosis of circulating lymphocytes in rats with unilateral ureteral obstruction?
Does angiotensin II blockade with losartan or enalapril reduce apoptosis of circulating lymphocytes in rats with unilateral ureteral obstruction?
Angiotensin II blockade with losartan or enalapril significantly reduces circulating lymphocyte apoptosis induced by unilateral ureteral obstruction in rats.
Answers are grounded in this paper and cite their sources.
May attenuate lymphocyte apoptosis in rat UUO models; leaves open translation to human obstructive nephropathy.
BACKGROUND: Unilateral ureteral obstruction (UUO) could induce increased renal angiotensin II (ANG II), which enhances apoptosis of renal tubular cells and renal tissue loss. Systemic ANG II is also increased in UUO. There are no data available about whether UUO can induce apoptosis of circulating lymphocytes or not. METHODS: UUO or sham-operated male Wistar rats (n = 8 in each group) were fed a drinking solution containing water, angiotensin II receptor type 1 antagonist (ARA; losartan, 500 mg/L) or angiotensin-converting enzyme inhibitor (ACEI; enalapril: 200 mg/L) for 1 day or 7 days. Blood samples were collected and circulating lymphocyte cells were separated. The apoptotic cells were detected by in situ terminal deoxynucleotidyl transferase (TdT assay)-mediated digoxigenin/antidigoxigenin conjugated fluorescein method and counted under a fluorescence microscope. The apoptotic index was calculated. RESULTS: UUO caused marked increases in the apoptotic index of circulating lymphocytes in UUO rats at both 1 day and 7 days when compared with the respective sham groups (P < 0.001). Neither ARA nor ACEI treatment had an effect on the apoptotic index values in the UUO rats at 1 day. In the UUO rats at 7 days, the apoptosis of circulating lymphocytes was markedly decreased from 29.2 +/- 2.7% to 11.9 +/- 2.7% (P < 0.01) in the ARA-treated rats and to 7.6 +/- 2.7% (P < 0.001) in the ACEI-treated rats. CONCLUSION: UUO, via stimulation of ANG II, could promptly enhance apoptosis of circulating lymphocytes. The apoptosis persisted throughout the 7 days of the study. Prolonged UUO would impair lymphocyte cell immunity and the host defense mechanism. Continuous treatment with either ARA or ACEI could abrogate ANG II-stimulated circulating lymphocyte apoptosis.
No takes yet. Share an insight, caveat, or question.
Eiam‐Ong et al. (2005) studied Unilateral ureteral obstruction. Angiotensin II receptor type 1 antagonist (losartan) or ACE inhibitor (enalapril) vs. Water (untreated UUO) was evaluated on Apoptotic index of circulating lymphocytes at 7 days (p=<0.01). In rats with unilateral ureteral obstruction, 7-day treatment with losartan or enalapril significantly reduced the circulating lymphocyte apoptotic index from 29.2% to 11.9% and 7.6%, respectively.
Synapse has enriched 10 related papers. Consider them for comparative context: