Key result
Quercetin and naringenin significantly increased cultured adult rat cardiomyocyte survival against daunorubicin-induced toxicity, demonstrating better protective effects than trolox (p<0.001).
Why the study?
Do flavonoids (quercetin, naringenin, pycnogenol) prevent daunorubicin-induced cardiotoxicity in cultured adult rat cardiomyocytes?
Do flavonoids (quercetin, naringenin, pycnogenol) prevent daunorubicin-induced cardiotoxicity in cultured adult rat cardiomyocytes?
p-value: p=<0.001
In an in vitro model, flavonoids such as quercetin and naringenin provided significant protection against daunorubicin-induced cardiotoxicity.
Hypothesis-generating for flavonoid cardioprotection in anthracycline models; leaves open clinical translation from this in vitro animal study.
Flavonoids are an ubiquitous group of polyphenolic substances with varied chemical structures present in foods of plant origin and act as free radical scavenging and chelating agents with a variety of biological activities. Using a model of spontaneously beating, cultured adult rat cardiomyocytes, this study examined the cardioprotective role of quercetin, naringenin, pycnogenol and a model antioxidant, trolox, against daunorubicin-induced toxicity. Cardiomyocyte protection was assessed by MTT test and extracellular lactate dehydrogenase detection. Protection of cardiomyocytes was concentration/dose dependent for quercetin > naringenin > pycnogenol > trolox. Quercetin (10(-4)-10(-6) mol/L) after 24 h of co-incubation with daunorubicin significantly increased the cardiomyocyte survival in all tested concentrations (p < 0.001). The cytoprotective effect of naringenin (10(-4)-10(-6) mol/L) was similar to those of quercetin (p < 0.001 and p < 0.01, respectively). Pycnogenol was the least effective of the flavonoids studied. On the other hand, all tested flavonoids had significantly better protective effects than trolox. The leakage of lactate dehydrogenase induced by daunorubicin was also prevented by the studied compounds and was in accordance with their cytoprotective activity.
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Mojžišová et al. (2006) studied Daunorubicin-induced cardiotoxicity. Flavonoids (quercetin, naringenin, pycnogenol) vs. Trolox was evaluated on Cardiomyocyte survival and extracellular lactate dehydrogenase leakage (p=<0.001). Quercetin and naringenin significantly increased cultured adult rat cardiomyocyte survival against daunorubicin-induced toxicity, demonstrating better protective effects than trolox (p<0.001).
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