Key result
Cold storage of dog renal cortical cytosol at 0 degrees C for 7 days completely converted renin from a 40,000 molecular weight form to a 60,000 molecular weight form.
Population
Dog kidney (soluble fraction of renal cortical homogenate and renin extracted from isolated renin granules)
Comparison
Cold storage at 0 degrees C for up to 7 days vs Renin in granules kept at 0 degrees C or baseline
Design
Preclinical
Follow-up
7 days
Authors
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Storage-induced renin conversion in canine cytosol warrants assay caution; leaves open in vivo relevance to human renin forms.
Cold storage of dog kidney cytosol at 0 degrees C induces the conversion of renin to a higher-molecular-weight form, a process dependent on a renin-binding substance.
Kawamura et al. (1980) studied this question. Cold storage (0 degrees C) was evaluated on Molecular weight conversion of renin. Cold storage of dog renal cortical cytosol at 0 degrees C for 7 days completely converted renin from a 40,000 molecular weight form to a 60,000 molecular weight form.
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