Key result
Extraction of canine renal cortical tissue yielded two high molecular weight renin forms (65K and 55K) that converted to a 41K low molecular weight form over 2 days of storage at 4°C.
Population
Canine renal cortical tissue
Design
Preclinical
Follow-up
2 days
Authors
Loading...
Caution against altering clinical renin assays; leaves open multistep proteolytic processing in canine models for targeted biochemical follow-up.
The identification of a 55K intermediate form of renin suggests that the proteolytic processing of high molecular weight renin to its low molecular weight form involves multiple steps.
Potter et al. (1979) studied this question. Extraction of canine renal cortical tissue at pH 7.4 with protease inhibitors was evaluated on Renin activity and molecular weight forms. Extraction of canine renal cortical tissue yielded two high molecular weight renin forms (65K and 55K) that converted to a 41K low molecular weight form over 2 days of storage at 4°C.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: