What is the clinical evidence from this study?

Study design: Other. Intervention: Calcium ionophore A-23187 vs. Rings without valves. Primary outcome: Endothelium-dependent relaxation.

What question did this study set out to answer?

To evaluate endothelium-dependent relaxation induced by the calcium ionophore A-23187 in isolated canine saphenous vein segments with and without valves.

February 1, 2001AJP Heart and Circulatory PhysiologyOpen Access

Inhibitory effect of valves on endothelium-dependent relaxations to calcium ionophore in canine saphenous vein

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Key result

Endothelium-dependent relaxation to A-23187 is significantly reduced in regions of canine saphenous veins with valves compared with the nonvalvular venous wall.

Population

Isolated canine saphenous veins

Comparison

Calcium ionophore A-23187 (10(-9)-10(-6) M) vs Rings with valves versus rings without valves

Design

Preclinical

Authors

DEDaihiko EguchiFukuoka City Hospital ZKZvonimir S. KatušićVascular / Pulmonary Vascular

Discussion

Member takes

Implication

Regional endothelial impairment in canine venous valves may affect graft function; leaves open human relevance and mechanisms.

Key Points

To evaluate endothelium-dependent relaxation induced by the calcium ionophore A-23187 in isolated canine saphenous vein segments with and without valves.
Measured isometric force recordings and cGMP levels in isolated canine saphenous vein rings with and without valves precontracted with U-46619.
Tested vasorelaxation responses to A-23187, forskolin, and DEA-NONOate alongside inhibitors targeting nitric oxide synthase (L-NAME), cyclooxygenase (indomethacin), and soluble guanylate cyclase (ODQ).
Endothelium-dependent relaxation to A-23187 was significantly reduced in rings with valves compared with rings without valves, whereas responses to forskolin and DEA-NONOate were identical across groups.
L-NAME and indomethacin each partially reduced A-23187-induced relaxation in nonvalvular rings, whereas L-NAME had no effect and indomethacin completely abolished relaxation in valvular rings.
ODQ abolished A-23187-stimulated cGMP increases but had no effect on vasorelaxation in either valvular or nonvalvular rings.

Structured PICO

Population

Isolated canine saphenous veins

Intervention

Calcium ionophore A-23187 (10(-9)-10(-6) M)

Comparator

Rings with valves versus rings without valves

Outcome

Endothelium-dependent relaxation (isometric force recordings) and cGMP measurementssurrogate

Endothelium-dependent relaxation to A-23187 is reduced in valvular regions of canine saphenous veins, likely due to lower nitric oxide production in endothelial cells of valvular segments.

Cite This Study

Eguchi et al. (2001) studied this question. Calcium ionophore A-23187 vs. Rings without valves was evaluated on Endothelium-dependent relaxation. Endothelium-dependent relaxation to A-23187 is significantly reduced in regions of canine saphenous veins with valves compared with the nonvalvular venous wall.

synapsesocial.com/papers/6a9baf4e0b0288a0688fec34 https://doi.org/10.1152/ajpheart.2001.280.2.h892

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Inhibitory effect of valves on endothelium-dependent relaxations to calcium ionophore in canine saphenous vein

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Inhibitory effect of valves on endothelium-dependent relaxations to calcium ionophore in canine saphenous vein

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider