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August 1, 1990Journal of Cardiovascular Pharmacology

Release of Endothelium-Derived Relaxing Factors from Canine Cardiac Valves

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Key result

Intact cardiac valvular endothelium significantly depressed PGF2 alpha-induced contraction in canine coronary arteries, suggesting basal release of vasodilatory prostanoids and relaxing factor.

Population

Endothelium-denuded canine coronary arteries and small segments of canine cardiac valve with intact…

Comparison

Pharmacological stimulation and inhibition using… vs Endothelium-denuded coronary arteries in the…

Design

Preclinical

Authors

DKDavid D. KuUniversity of Alabama at BirminghamJNJulie M. NelsonPearson (United States)JCJames CaulfieldBoston University

Discussion

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Implication

Valvular endothelium does not modulate coronary contractility in this model; leaves open its in vivo role in vasoregulation.

Key Points

  • To investigate whether intact endothelial cells on canine cardiac valves continuously release vasodilatory prostanoids and endothelium-derived relaxing factor.
  • Mounted endothelium-denuded canine coronary arterial rings in organ chambers to bioassay contractile and relaxant responses.
  • Inserted small segments of canine cardiac valve (20–30 mm²) with intact endothelium into the arterial ring preparations in the presence or absence of pharmacological modulators.
  • Valvular endothelium significantly depressed prostaglandin F2 alpha-induced contractions by 70% in magnitude and increased ED50 by 216% relative to control (5.52 ± 0.49 g; ED50 of 1.18 ± 0.02 μM), an effect attenuated by 5 μM indomethacin to a 38% decrease.
  • Acetylcholine induced concentration-dependent arterial relaxation reaching -59.9 ± 1.6% (n=7), which indomethacin reduced to 40.4 ± 5.5% (n=13), hemoglobin further diminished to -16.0 ± 7.7% (n=14), and superoxide dismutase augmented to -81.3 ± 9.4% (n=11).

Structured PICO

P
Population
Endothelium-denuded canine coronary arteries and small segments of canine cardiac valve (20-30 mm2) with intact endothelium
I
Intervention
Pharmacological stimulation and inhibition using acetylcholine (0.1-30 microM), indomethacin (5 microM), hemoglobin (3 microM), and superoxide dismutase (20 units/ml)
C
Comparator
Endothelium-denuded coronary arteries in the absence of valvular endothelium
O
Outcome
Magnitude and sensitivity of coronary artery contractile response to KCl and PGF2 alpha, and relaxation response to acetylcholinesurrogate

Canine cardiac valvular endothelium continuously releases vasodilatory prostanoids and endothelium-derived relaxing factor, which can modulate coronary artery tone.

Cite This Study

Ku et al. (1990) studied this question. Intact cardiac valvular endothelium vs. Absence of valvular endothelium was evaluated on PGF2 alpha-induced contraction. Intact cardiac valvular endothelium significantly depressed PGF2 alpha-induced contraction in canine coronary arteries, suggesting basal release of vasodilatory prostanoids and relaxing factor.

synapsesocial.com/papers/6a6bbd1bec7caa0664b38eefhttps://doi.org/10.1097/00005344-199008000-00006
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Endothelium‐dependent hyperpolarization of canine coronary smooth muscle1988 · 776 citations
  2. 2Release and properties of endothelium‐derived relaxing factor (EDRF) from endothelial cells in culture1985 · 227 citations
  3. 3Bioassay of endothelium-derived relaxing factor(s): inactivation by catecholamines1985 · 152 citations
  4. 4Endothelial Cells as Mediators of Vasodilation of Arteries1984 · 324 citations
  5. 5Inhibitory effect of valves on endothelium-dependent relaxations to calcium ionophore in canine saphenous vein2001 · 3 citations