Key result
Mutation of the F380 residue in hSlo channels to isoleucine or tyrosine reduced unitary conductance from 294 pS to 92 pS and 166 pS, respectively, highlighting its critical role in gating.
Population
Human embryonic kidney cell line (HEK293) expressing wild-type and mutant (F380I, F380Y) hSlo channels
Comparison
Mutation of F380 in the S6 region to isoleucine… vs Wild-type hSlo channels
Design
Preclinical
Authors
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No immediate clinical implications from this animal model; extends molecular understanding of BK channel gating.
The conserved F380 residue in the S6 region of BKCa channels is critical for gating and permeation, likely lining the conduction pathway.
Lippiat et al. (2000) studied this question. F380 mutations (F380I, F380Y) in hSlo channels vs. Wild-type hSlo channels was evaluated on Unitary conductances. Mutation of the F380 residue in hSlo channels to isoleucine or tyrosine reduced unitary conductance from 294 pS to 92 pS and 166 pS, respectively, highlighting its critical role in gating.
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