What does this research mean for the field?

An optimized extraction protocol coupled with HPLC-DAD provides a rapid and robust method for the simultaneous quantification of carnosine and anserine across diverse meat matrices, revealing distinct dipeptide profiles such as high carnosine in pork and high anserine in white-feather Nin-Kaset chicken. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To develop and validate a simplified extraction method coupled with HPLC-DAD for the simultaneous quantification of carnosine and anserine in various meat types.

September 6, 2026Methods and ProtocolsOpen Access

Quantification of Carnosine and Anserine in Diverse Meat Matrices via Optimized Extraction and Validated HPLC-DAD

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Authors

CKChalita Kaewkhow CCChokwan Chumang PPPiboon Pantu

Discussion

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Overview

Analytical validation study demonstrates accurate quantification of carnosine and anserine across diverse meats, indicating robust utility for food authentication and nutritional profiling.

Key Points

To develop and validate a simplified extraction method coupled with HPLC-DAD for the simultaneous quantification of carnosine and anserine in various meat types.
Optimized dipeptide extraction efficiency across meat matrices (Thai native black-bone chicken, commercial broiler, pork, beef, and buffalo) by adjusting solution pH.
Validated the HPLC-DAD analytical procedure for linearity, sensitivity (limits of detection and quantification), recovery, and repeatability.
The method achieved linear response across 1–200 µg mL−1 (r² ≥ 0.9990), with recoveries of 96.64–102.68% and repeatability relative standard deviations of 0.70–14.18%.
Pork demonstrated the highest carnosine concentration at 3.59 ± 0.19 mg g−1, whereas white-feather Nin-Kaset black-bone chicken exhibited the highest anserine concentration at 6.77 ± 0.40 mg g−1.

Cite This Study

Kaewkhow et al. (2026) studied this question.

synapsesocial.com/papers/6a9d1e7228139818eab213f2 https://doi.org/10.3390/mps9050131

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September 6, 2026Methods and ProtocolsOpen Access

Quantification of Carnosine and Anserine in Diverse Meat Matrices via Optimized Extraction and Validated HPLC-DAD

View Full Paper

Ask AI

Bookmark

Authors

CKChalita Kaewkhow CCChokwan Chumang PPPiboon Pantu

Discussion

Member takes

Overview

Analytical validation study demonstrates accurate quantification of carnosine and anserine across diverse meats, indicating robust utility for food authentication and nutritional profiling.

Key Points

To develop and validate a simplified extraction method coupled with HPLC-DAD for the simultaneous quantification of carnosine and anserine in various meat types.
Optimized dipeptide extraction efficiency across meat matrices (Thai native black-bone chicken, commercial broiler, pork, beef, and buffalo) by adjusting solution pH.
Validated the HPLC-DAD analytical procedure for linearity, sensitivity (limits of detection and quantification), recovery, and repeatability.
The method achieved linear response across 1–200 µg mL−1 (r² ≥ 0.9990), with recoveries of 96.64–102.68% and repeatability relative standard deviations of 0.70–14.18%.
Pork demonstrated the highest carnosine concentration at 3.59 ± 0.19 mg g−1, whereas white-feather Nin-Kaset black-bone chicken exhibited the highest anserine concentration at 6.77 ± 0.40 mg g−1.

Cite This Study

Kaewkhow et al. (2026) studied this question.

synapsesocial.com/papers/6a9d1e7228139818eab213f2 https://doi.org/10.3390/mps9050131

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