Angiopoietin-like protein 4 (ANGPTL4) is an inhibitor of endothelial lipase (EL) while the ANGPTL4/8 complex has reduced EL-inhibitory activity

Figures

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  Figure 1. ANGPTL3 and ANGPTL3/8 inhibition of EL. (A) The ability of ANGPTL3 or ANGPTL3/8 to inhibit EL at 37 °C was assessed using an EL stable expression cell line and phospholipase A1 selective substrate. Fluorescence was monitored with an excitation wavelength of 485 nm and an emission wavelength of 516 nm. Readings were taken at 1 and 30 min, with the 1-minute reading subtracted from the 30-minute reading to correct for background fluorescence. Results are shown as the mean ± SD (n = 6 from 3 independent experiments). (B) The ability of ANGPTL3 or ANGPTL3/8 to inhibit EL was assessed as in Figure 1A except at 22 °C. Results are shown as the mean ± SD (n = 6 from 3 independent experiments).

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  Figure 2. ANGPTL4 and ANGPTL4/8 inhibition of EL. (A) The ability of ANGPTL4 or ANGPTL4/8 to inhibit EL at 37 °C was assessed using an EL stable expression cell line and phospholipase A1 selective substrate. Fluorescence was monitored with an excitation wavelength of 485 nm and an emission wavelength of 516 nm. Readings were taken at 1 and 30 min, with the 1-minute reading subtracted from the 30-minute reading to correct for background fluorescence. Results are shown as the mean ± SD (n = 4 from 2 independent experiments). (B) The ability of ANGPTL4 or ANGPTL4/8 to inhibit EL was assessed as in Figure 2A except at 22 °C. Results are shown as the mean ± SD (n = 8 from 4 independent experiments).

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  Figure 3. ANGPTL4 and ANGPTL4/8 inhibition of recombinant EL protein activity. The ability of ANGPTL4 or the ANGPTL4/8 complex to inhibit human recombinant EL protein activity was assessed using phospholipase A1 selective substrate. Fluorescence was monitored with an excitation wavelength of 485 nm and an emission wavelength of 516 nm. Readings were taken at 1 and 30 min, with the 1-minute reading subtracted from the 30-minute reading to correct for background fluorescence. Results are shown as the mean ± SD (n = 4 from 2 independent experiments).