Identification of a novel foot-and-mouth disease virus specific T-cell epitope with immunodominant characteristics in cattle with MHC serotype A31

Q: What is the clinical evidence from this study?

Population: Foot-and-mouth disease virus (FMDV) infection (n=4). Intervention: FMDV overlapping pentadecapeptides vs. Control peptide and medium alone. Primary outcome: T-cell proliferation and IFN-γ production.

Wilhelm Gerner; B. Veronica Carr; Karl‐Heinz Wiesmüller; Eberhard Pfaff; Armin Saalmüller; Bryan Charleston

doi:10.1051/vetres:2007016

May 30, 2007Veterinary ResearchOpen Access

Identification of a novel foot-and-mouth disease virus specific T-cell epitope with immunodominant characteristics in cattle with MHC serotype A31

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Key result

A novel T-cell epitope located on the FMDV structural protein 1D (VP1, amino acids 66-80) induced proliferation and interferon-gamma production in all investigated cattle with MHC serotype A31.

Structured PICO

P

Population

4 male Holstein cattle with MHC serotype A31, experimentally infected with FMDV at 6 months of age and followed for up to 21 weeks.

I

Intervention

In vitro stimulation of peripheral blood mononuclear cells (PBMC) with 442 overlapping pentadecapeptides covering the entire FMDV polyprotein sequence.

C

Comparator

Cells in medium alone and cells incubated with a 15-mer peptide from the Core protein of classical swine fever virus.

O

Outcome

Lymphocyte proliferation (measured by 3H-thymidine incorporation) and Interferon-gamma production (measured by ELISPOT).surrogate

The study identifies a novel immunodominant T-cell epitope on the FMDV structural protein 1D (VP1) in cattle with MHC serotype A31, which may inform future vaccine design.

Limitations

Small sample size of only four animals
Animals were infected only once, which may have contributed to a rapid decline of memory T-cells
Only one MHC serotype (A31) was investigated
Small sample size (n=4)
Animals were infected only once, which may have contributed to a rapid decline of memory T-cells in circulation
All animals shared the MHC serotype A31, limiting generalizability to other MHC types

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Implication

May guide FMDV T-cell vaccine design in A31 cattle; leaves open validation across MHC types and species.

Abstract

To identify foot-and-mouth disease virus (FMDV) specific T-cell epitopes within the entire polyprotein sequence of the virus, 442 overlapping pentadecapeptides were tested in proliferation assays using lymphocytes from cattle experimentally infected with FMDV. Four months post-infection cells from all investigated animals (n = 4) responded by proliferation and interferon-gamma production to a peptide located on the structural protein 1D (VP1), amino acid residues 66-80. Major histocompatibility complex (MHC) serotyping of the investigated cattle indicated that all animals shared the MHC serotype A31 which comprises the class II allele DRB3 0701. This may explain the common recognition of this newly discovered epitope. Responses to other peptides could only be observed in one animal and rapidly declined during the time course of the study. These observations point to an immunodominant role of this epitope located on the protein 1D in cattle with MHC serotype A31.

Discussion

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Cite This Study

Gerner et al. (2007) studied Foot-and-mouth disease virus (FMDV) infection (n=4). FMDV overlapping pentadecapeptides vs. Control peptide and medium alone was evaluated on T-cell proliferation and IFN-γ production. A novel T-cell epitope located on the FMDV structural protein 1D (VP1, amino acids 66-80) induced proliferation and interferon-gamma production in all investigated cattle with MHC serotype A31.

synapsesocial.com/papers/6a9e7384065205258095e935 https://doi.org/10.1051/vetres:2007016

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Identification of a novel foot-and-mouth disease virus specific T-cell epitope with immunodominant characteristics in cattle with MHC serotype A31

Structured PICO

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Identification of a novel foot-and-mouth disease virus specific T-cell epitope with immunodominant characteristics in cattle with MHC serotype A31

Structured PICO

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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