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July 25, 2011The Journal of General PhysiologyOpen Access

R1 in the Shaker S4 occupies the gating charge transfer center in the resting state

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Key result

Mutational analysis and Zn(2+) binding assays indicate that the R1 residue in the Shaker S4 segment occupies the gating charge transfer center in the resting state.

Population

Shaker channels with mutations (I287H, I287H+R1H, I287H+A359H)

Design

Preclinical

Authors

MLMeng‐Chin LinUniversity of California, Los AngelesJHJui-Yi HsiehIncyte (United States)AMAllan F. MockUniversity of California, Los Angeles

Discussion

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Implication

Refines voltage sensor models in research; leaves open relevance to human cardiac channels.

Structured PICO

P
Population
Shaker channels with mutations (I287H, I287H+R1H, I287H+A359H)
I
Intervention
Extracellular Zn(2+) application and voltage prepulses
O
Outcome
Effect of extracellular Zn(2+) on channel activity (slow component of activation, A(slow,max))surrogate

The study provides biophysical evidence that the R1 residue in the Shaker S4 segment occupies the gating charge transfer center in the resting conformation.

Cite This Study

Lin et al. (2011) studied this question. I287H mutation and Zn(2+) application was evaluated on Zn(2+) binding and slow component of activation (A(slow,max)). Mutational analysis and Zn(2+) binding assays indicate that the R1 residue in the Shaker S4 segment occupies the gating charge transfer center in the resting state.

synapsesocial.com/papers/6a9e97cff76ddda103f9acd6https://doi.org/10.1085/jgp.201110642
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Two atomic constraints unambiguously position the S4 segment relative to S1 and S2 segments in the closed state of Shaker K channel2007 · 169 citations
  2. 2Optical detection of rate-determining ion-modulated conformational changes of the ether-à-go-go K + channel voltage sensor2005 · 42 citations
  3. 3Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinement2010 · 236 citations
  4. 4Voltage Sensitivity and Gating Charge in Shaker and Shab Family Potassium Channels1999 · 173 citations
  5. 5Mg2+ Modulates Voltage-Dependent Activation in Ether-à-Go-Go Potassium Channels by Binding between Transmembrane Segments S2 and S32000 · 63 citations