Plasma cytokines do not reflect expression of pro‐ and anti‐inflammatory cytokine mRNA at organ level after cardiopulmonary bypass in neonatal pigs*
Key result
Cardiopulmonary bypass in neonatal pigs induced significant changes in organ cytokine mRNA expression at 0.5 and 4 hours that were poorly reflected by plasma cytokine levels.
Why the study?
Do plasma cytokine levels reflect organ-level cytokine mRNA expression after cardiopulmonary bypass in neonatal pigs?
Population
26 neonatal piglets (17-19 days old)
Comparison
Cardiopulmonary bypass procedure vs Sham procedure (sternotomy only)
Design
Preclinical, allocated
Follow-up
0.5 h or 4 h post-CPB
Authors
Discussion
Loading...
Member takes
Implication
Plasma cytokines should not proxy organ inflammation after neonatal CPB; leaves open need for tissue-level assessment in translational studies.
Structured PICO
Do plasma cytokine levels reflect organ-level cytokine mRNA expression after cardiopulmonary bypass in neonatal pigs?
P
Population
26 piglets (17-19 days old) allocated to a sham procedure or cardiopulmonary bypass and observed for 0.5 or 4 hours.
I
Intervention
Cardiopulmonary bypass (CPB) procedure (sternotomy, 120 min CPB with 60-min aortic cross-clamp)
C
Comparator
Sham procedure (sternotomy only)
O
Outcome
Plasma levels of IL-1beta, IL-6, IL-8 and IL-10 and mRNA expression of TNF-alpha, IL-1beta, IL-6, IL-8, IL-10 and iNOS in organssurrogate
Plasma cytokine levels do not accurately reflect organ-level inflammatory mRNA expression following cardiopulmonary bypass in a neonatal pig model.
Cite This Study
Christensen et al. (2003) studied Cardiac surgery and cardiopulmonary bypass (n=26). Cardiopulmonary bypass (CPB) vs. Sham procedure (sternotomy only) was evaluated on Plasma levels of IL-1beta, IL-6, IL-8 and IL-10 and mRNA expression of TNF-alpha, IL-1beta, IL-6, IL-8, IL-10 and iNOS in organs. Cardiopulmonary bypass in neonatal pigs induced significant changes in organ cytokine mRNA expression at 0.5 and 4 hours that were poorly reflected by plasma cytokine levels.