Key result
A tilt test procedure in anesthetized, normotensive rats yields reproducible orthostatic hypotensive responses for evaluating the effects of test substances on blood pressure and heart rate.
This paper describes a relatively simple and reproducible tilt test procedure in anesthetized rats for evaluating drug-induced orthostatic hypotension.
Provides a reproducible rat model for orthostatic hypotension screening; leaves open translation to conscious animals and humans.
Postural-change-induced (orthostatic) hypotension is defined as an excessive drop in arterial blood pressure occurring when moving toward an upright position. This side effect, which may limit the therapeutic use of some agents, can occur with drugs, such as adrenoceptor blockers and vasodilators, that dampen sympathetic reflex activity. Described in this unit is a procedure for evaluating the effects of test substances on the changes in blood pressure and heart rate that occur in an anesthetized, normotensive rat during a tilting challenge (head-up position). In addition to being a relatively simple technique, this assay yields reproducible orthostatic hypotensive responses and allows for the investigation, in the same preparation, of several ascending doses of a test substance. Examples of results obtained with prazosin, an α1-adrenoceptor antagonist that is notorious for causing orthostatic hypotension, are provided for illustrative purposes.
No takes yet. Share an insight, caveat, or question.
Guillaume et al. (2008) studied Orthostatic hypotension. Test substances (e.g., prazosin) during a tilting challenge was evaluated on Changes in blood pressure and heart rate. A tilt test procedure in anesthetized, normotensive rats yields reproducible orthostatic hypotensive responses for evaluating the effects of test substances on blood pressure and heart rate.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: