Key result
HPLC/APCI-MS/MS analysis of amiodarone incubated with rat liver microsomes identified structures for two proposed metabolites and four impurities.
This study provides structural characterization of two metabolites and four impurities of the anti-arrhythmic drug amiodarone using mass spectrometry.
Supports metabolite identification in vitro; leaves open human pharmacokinetic relevance.
Using the high performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS) technique, together with established trends from the literature, the structures of metabolites and impurities of amiodarone, an anti-arrhythmic drug, have been assigned. By comparing analyses of products of incubation with rat liver microsomes with controls in which glucose 6-phosphate dehydrogenase was omitted, metabolites could be distinguished from impurities. Structures for the two proposed metabolites and four impurities are proposed.
No takes yet. Share an insight, caveat, or question.
Myung et al. (2000) studied this question. Amiodarone vs. Controls without glucose 6-phosphate dehydrogenase was evaluated on Identification of metabolites and impurities. HPLC/APCI-MS/MS analysis of amiodarone incubated with rat liver microsomes identified structures for two proposed metabolites and four impurities.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: