March 1, 1983Canadian Journal of Physiology and Pharmacology
Rapid high-performance liquid chromatographic analysis of amiodarone and N-desethyl-amiodarone in serum
Key result
Rapid reverse-phase HPLC assay quantifies serum amiodarone with ~97% accuracy.
Population
Serum samples for measurement of amiodarone (n=37) and N-desethyl-amiodarone (n=32)
Design
Other
Authors
Discussion
Loading...
Member takes
Implication
May support amiodarone therapeutic monitoring; leaves open prospective outcome validation.
Structured PICO
P
Population
Serum samples for measurement of amiodarone (n=37) and N-desethyl-amiodarone (n=32)
I
Intervention
High-performance liquid chromatographic (HPLC) assay with ultraviolet-visible detection
O
Outcome
Accuracy and coefficient of variation of the HPLC assaysurrogate
A rapid and reliable HPLC assay was developed for the accurate measurement of the antiarrhythmic drug amiodarone and its metabolite in serum.
Cite This Study
Brien et al. (1983) studied this question. High-performance liquid chromatographic (HPLC) assay was evaluated on Assay accuracy and sensitivity. A rapid reverse-phase HPLC assay accurately measured amiodarone (96.9% accuracy) and N-desethyl-amiodarone (99.8% accuracy) in serum with a quantitative sensitivity limit of 0.10 micrograms/mL.