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June 1, 1970JNCI Journal of the National Cancer Institute

Attenuation of Marek's Disease Virus and Study of Its Properties in Two Different Cell Cultures

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Key result

Marek's disease virus lost pathogenicity and changed cytopathology after long cell-culture passage in duck embryo fibroblasts, with no cell-free virus recovered compared to chicken embryo fibroblasts.

Population

Duck embryo fibroblast and chicken embryo fibroblast cultures infected with Marek's disease virus

Design

Preclinical

Authors

KNK. Nazerian

Discussion

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Overview

May affect MDV attenuation models in vitro; leaves open cellular determinants of pathogenicity for in vivo validation.

Structured PICO

P
Population
Duck embryo fibroblast (DEF) and chicken embryo fibroblast (CEF) cultures infected with Marek's disease virus (MDV)
I
Intervention
Long cell-culture passage in DEF and propagation in CEF by cocultivation
O
Outcome
Cytopathology, pathogenicity, and viral properties (cell-free virus recovery, microplaque morphology)

Marek's disease virus exhibits different cytopathology and cell-free virus recovery depending on whether it is cultured in duck or chicken embryo fibroblasts.

Cite This Study

K. Nazerian (1970) studied Marek's disease virus (in vitro). Duck embryo fibroblast (DEF) culture vs. Chicken embryo fibroblast (CEF) culture was evaluated on Cytopathology and pathogenicity of the virus. Marek's disease virus lost pathogenicity and changed cytopathology after long cell-culture passage in duck embryo fibroblasts, with no cell-free virus recovered compared to chicken embryo fibroblasts.

synapsesocial.com/papers/6aa02e5cf3f976aaa61339e7https://doi.org/10.1093/jnci/44.6.1257
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Suspension culture of Marek’s disease virus and evaluation of its immunological effects2018 · 11 citations
  2. 2Replication of Marek's Disease Virus in Cell Cultures Derived from Genetically Resistant Chickens1974 · 12 citations
  3. 3Macrophage Restriction of Marek's Disease Virus Replication and Lymphoma Cell Proliferation1979 · 45 citations
  4. 4Further characterization of marek's disease virus‐infected lymphocytes. I. In vivo infection1984 · 130 citations
  5. 5Characterizing the Molecular Basis of Attenuation of Marek's Disease Virus via In Vitro Serial Passage Identifies De Novo Mutations in the Helicase-Primase Subunit Gene UL5 and Other Candidates Associated with Reduced Virulence2014 · 26 citations