The effect of n-butanol subfractions of Adansonia digitata and Corchorus olitorius on gamma-irradiation-induced haematological alterations and Fe3+and CO2 dysregulation

• The HPLC fingerprinting of the n-butanol subfractions of A. digitata and C. olitorius revealed the presence of bioactive constituents such as catechin, luteolin, astragalin, and quercetin. • The Leaves-based diets of adansonia digitata and corchorus olitorius have been found to be protective in function when feed on rats. Radiation increases free radical from sub-toxic to toxic concentration in rats. Free radicals attack and transformed haemoglobin from biconcave to echinocyte structure (sickling), leading to haematological alteration.This study investigated the effects of n-butanol subfractions 1, 2, 3 and 4 of A. digitata and 1, 2 and 3 of C. olitorius leaves against irradiation-induced haematological alterations in Rats. A total of 54 rats (198 ± 5.00 g) grouped into 9 of 6 each; Group i were placed on animal feed and clean water only. Groups ii-ix were exposed to irradiation and groups ii, iii, iv, v, vi, vii, viii and ix were provided-with clean water only, 1000 mg/kg body weight subfractions 1, 2, 3 and 4 of A. digitata and 1, 2 and 3 of C. olitorius respectively for 7 days. HPLC fingerprinting of the subfractions of both leaves contained catechin, luteolin, astragalin, eleagnin, quercetin, tocopherol and others. Irradiation significantly ( p < 0.05) decreased haemoglobin concentration (HBC), red blood cell count (RBC), platelets (PLT) and white blood cell (WBC) of rats, when compared with the control. Administration of the subfractions of A. digitata and C. olitorius leaves significantly ( p < 0.05) increased HBC, RBC, PLT and WBC when compared with non-administered groups. The concentration of bilirubin, Fe 3+ and CO 2 significantly ( p < 0.05) increased in the irradiated rats, but were attenuated by the n-butanol subfractions of both plants. n-butanol subfractions of Adansonia digitata and Cochorous olitorius contained bioactive constituents that are heamato-protective.