Key result
RAG-1 and RAG-2 gene transfer fails to activate immunoglobulin gene recombination in some pre-B-cell lines.
Population
Abelson virus-transformed pre-B-cell lines that fail to undergo VH-to-DJH joining at an appreciable frequency
Comparison
Introduction of RAG-1 and RAG-2 genes via… vs Control transfections or unfused parental cell…
Design
Preclinical
Authors
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RAG-1/2 insufficiency in some pre-B lines cautions against sufficiency models; leaves open identification of required accessory regulators.
RAG-1 and RAG-2 are insufficient to direct all phases of immunoglobulin gene rearrangement in certain pre-B-cell lines, suggesting the requirement of additional factors.
Wang et al. (1993) studied Immunoglobulin gene rearrangement in Pre-B-Cell Lines. RAG-1 and RAG-2 gene transfer vs. Control cell lines was evaluated on Recombinase activity. Gene transfer and cell fusion experiments indicate that RAG-1 and RAG-2 are not sufficient to activate immunoglobulin gene recombination in at least some pre-B-cell lines.
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