What question did this study set out to answer?

Determine the molecular identity of the spaghetti-squash gene and evaluate its functional role in Drosophila cytokinesis.

June 1, 1991CellOpen Access

The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila

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Authors

RKRoger E. KaressCentre National de la Recherche Scientifique XCXiao-Jia ChangHarvard University KEKevin A. EdwardsIllinois State University

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Implication

Genetic analysis demonstrates that spaghetti-squash encodes myosin regulatory light chain required for cytokinesis in Drosophila, indicating its essential role in contractile ring function.

Key Points

Determine the molecular identity of the spaghetti-squash gene and evaluate its functional role in Drosophila cytokinesis.
Screened for mitotic mutants in Drosophila to isolate the cytokinesis-defective sqh1 allele and cloned the locus using transposon tagging.
Cloned the nonmuscle myosin regulatory light chain (MRLC-C) gene and quantified transcript abundance in sqh1 mutant backgrounds.
Evaluated phenotypic rescue of sqh1 defects via transposon excision and germline transformation with wild-type sqh transcription units.
Identified that the spaghetti-squash gene encodes MRLC-C, with sqh1 mutants exhibiting severely reduced levels of stable regulatory light chain transcript.
Transposon excision or wild-type sqh transgene expression completely rescued cytokinesis failure and associated mutant phenotypes in sqh1 flies.

Cite This Study

Karess et al. (1991) studied this question.

synapsesocial.com/papers/6aa1f70c0c07007fbcb2adc3 https://doi.org/10.1016/0092-8674(91)90013-o

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Implication

Genetic analysis demonstrates that spaghetti-squash encodes myosin regulatory light chain required for cytokinesis in Drosophila, indicating its essential role in contractile ring function.

The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila

Discussion

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Implication

Key Points

Cite This Study

The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila

Discussion

Member takes

Implication

Key Points

Cite This Study

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