Key result
PCR-RFLP analysis of the short fiber gene successfully differentiates HPS-causing FAdV-4 strains from non-HPS strains.
PCR-RFLP analysis of short fiber genes can distinguish FAdV-4 strains causing hydropericardium syndrome in chickens from other strains.
May enable strain-specific FAdV-4 identification in poultry diagnostics; leaves open validation and field translation.
The sequences of short fiber genes of the Fowl adenovirus serotype 4 (FAdV-4), including isolates from chickens with hydropericardium syndrome (HPS), in Japan, India, and Pakistan were compared. By phylogenetic analysis based on complete nucleotide sequences of this gene, FAdV-4 strains from HPS (HPS-FAdV-4) in Japan, India, and Pakistan fell into a different cluster from FAdV-4 strains not derived from HPS. Hydropericardium syndrome-FAdV-4 isolates were differentiated from other FAdV-4 strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using the enzyme the Alu I. The use of PCR-RFLP analysis of short fiber genes may be useful to distinguish among FAdV-4 strains.
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Mase et al. (2010) studied Hydropericardium syndrome in chickens. PCR-RFLP analysis of short fiber genes was evaluated on Differentiation of FAdV-4 strains. Phylogenetic and PCR-RFLP analysis of the short fiber gene successfully differentiated FAdV-4 strains causing hydropericardium syndrome in chickens from non-HPS strains.
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