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December 21, 2009DNA and Cell Biology

Electrotransfer of cDNA Coding for a Heterologous Prion Protein Generates Autoantibodies Against Native Murine Prion Protein in Wild-Type Mice

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Key result

Electrotransfer vaccination with human PrPc cDNA generates strong autoantibodies against native murine PrPc in mice.

Population

Wild-type mice

Comparison

Vaccination with cDNA coding for human PrP… vs Vaccination without using electrotransfer of DNA

Design

Preclinical

Follow-up

After three DNA injections

Authors

CACoralie AlexandrenneAWAnne WijkhuisenFDFatima Dkhissi

Discussion

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Overview

May inform PrP(c) vaccine strategies in animal models; leaves open translation to human prion disease trials.

Structured PICO

P
Population
Wild-type mice vaccinated with cDNA coding for human PrP(c) to investigate immune tolerance breaking.
I
Intervention
Vaccination with cDNA coding for human PrP(c), fused or unfused to a stimulatory T-cell epitope, using electrotransfer of DNA (three injections)
C
Comparator
Vaccination without using electrotransfer of DNA
O
Outcome
Development of immune response (IgG1 and IgG2a antibody titers against heterologous human PrP(c) and autoantibodies recognizing native murine PrP(c))surrogate

Genetic immunization with cDNA coding for a heterologous prion protein combined with electrotransfer successfully breaks immune tolerance in wild-type mice.

Cite This Study

Alexandrenne et al. (2009) studied Prion diseases. cDNA coding for human PrP(c) with electrotransfer vs. Without electrotransfer was evaluated on Immune response and autoantibody generation against native murine PrP(c). Vaccination of wild-type mice with cDNA coding for human PrP(c) using electrotransfer generated a strong humoral immune response and autoantibodies against native murine PrP(c).

synapsesocial.com/papers/6aa77bc541a3eb417e4f2584https://doi.org/10.1089/dna.2009.0940
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Polylactide-Coglycolide Microspheres CoEncapsulating Recombinant Tandem Prion Protein with CpG-Oligonucleotide Break Self-Tolerance to Prion Protein in Wild-Type Mice and Induce CD4 and CD8 T Cell Responses2007 · 55 citations
  2. 2A model-based prion vaccine protects a transgenic mouse line carrying a Gerstmann–Sträussler–Scheinker disease mutation2026 · 3 citations
  3. 3Dendritic Cell-Mediated-Immunization with Xenogenic PrP and Adenoviral Vectors Breaks Tolerance and Prolongs Mice Survival against Experimental Scrapie2009 · 21 citations
  4. 4The Murine B Cell Repertoire Is Severely Selected against Endogenous Cellular Prion Protein2005 · 31 citations
  5. 5CpG oligodeoxynucleotide-enhanced humoral immune response and production of antibodies to prion protein PrPSc in mice immunized with 139A scrapie-associated fibrils2007 · 51 citations