Key result
PAF drives in vitro platelet adhesion to endothelial cells only when PMNs are present.
Population
In vitro model of endothelial cells cultured in monolayer, platelets, and polymorphonuclear leukocytes (PMNs)
Comparison
Platelet-activating factor at concentrations… vs Lyso-PAF, absence of PMNs, or presence of PAF…
Design
Preclinical
Authors
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Does not support clinical use of PAF antagonists; leaves open their efficacy in leukocyte-dependent adhesion pending in vivo validation.
In vitro evidence shows that polymorphonuclear leukocytes are required for platelet-activating factor to induce platelet adhesion to vascular endothelial cells.
Hirafuji et al. (1991) studied this question. Platelet-activating factor (PAF) vs. Lyso-PAF or absence of PMNs was evaluated on Platelet adhesion to endothelial cells. Platelet-activating factor (PAF) induced marked platelet adhesion to endothelial cells in vitro only when polymorphonuclear leukocytes (PMNs) were present, a response suppressed by PAF antagonists.
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