What is the clinical evidence from this study?

Study design: Other. Intervention: Angiotensin 1-7 vs. Control/vehicle. Primary outcome: Aldosterone secretion (p=<0.01).

What does this research mean for the field?

Angiotensin (1-7) directly suppresses aldosterone secretion from adrenal glomerulosa cells in vitro and attenuates the low-salt diet-induced increase in aldosterone in vivo. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To determine whether angiotensin 1-7 directly modulates aldosterone secretion in adrenal glomerulosa cells both in vitro and in vivo.

June 1, 2016Hypertension

Angiotensin 1–7 Is a Negative Modulator of Aldosterone Secretion In Vitro and In Vivo

Q: What are the key findings of this study?

Continuous infusion of Angiotensin 1-7 in rats on a low-salt diet significantly attenuated the rise in aldosterone compared to vehicle (16.4 vs 27.6 ng/dL; P<0.01).

Q: What does this research mean for the field?

Angiotensin (1-7) directly suppresses aldosterone secretion from adrenal glomerulosa cells in vitro and attenuates the low-salt diet-induced increase in aldosterone in vivo. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Q: What question did this study set out to answer?

To determine whether angiotensin 1-7 directly modulates aldosterone secretion in adrenal glomerulosa cells both in vitro and in vivo.

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Key result

Angiotensin 1-7 infusion blunts aldosterone rise by ~41% versus vehicle in low-salt diet rats.

P<0.01

Why the study?

The direct effect of Angiotensin 1-7 on aldosterone secretion by adrenal glomerulosa cells was not fully understood.

Does Angiotensin 1-7 reduce aldosterone secretion in rat models?

Population

Isolated rat glomerulosa cells and rats on low-salt diet for 7 days

Comparison

Angiotensin 1-7 vs vehicle/control

Design

Preclinical in vitro and in vivo study

Follow-up

7 days

Authors

GSGabi SheferTel Aviv University YMYonit MarcusUniversité Côte d'Azur EKEsther KnollTel Aviv Sourasky Medical Center

Discussion

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Implication

Ang 1-7 may blunt aldosterone in low-salt rat models; leaves open any therapeutic role in human hypertension.

Key Points

To determine whether angiotensin 1-7 directly modulates aldosterone secretion in adrenal glomerulosa cells both in vitro and in vivo.
Immunofluorescence was performed to locate the Mas1 receptor in the subcapsular region of rat adrenal glands.
Isolated rat glomerulosa cells were stimulated with Ang II, ACTH, or potassium in the presence or absence of Ang 1-7 to evaluate in vitro aldosterone secretion.
Rats fed a low-salt diet for 7 days received continuous infusion of Ang 1-7 (576 μg/kg per day) or vehicle to measure plasma aldosterone and plasma renin activity.
In isolated glomerulosa cells, Ang 1-7 (10^-6 M) significantly attenuated aldosterone release stimulated by 10^-9 M Ang II from 189±11 to 33±3.6 pg/10^5 cells (P<0.001) and by 10^-8 M ACTH from 409±32.5 to 280±12.5 pg/10^5 cells (P<0.001), while potassium-stimulated secretion remained unaffected.
Continuous infusion of Ang 1-7 in salt-depleted rats significantly blunted the rise in plasma aldosterone (16.4±4.8 ng/dL vs. 27.6±5.3 ng/dL in vehicle controls; P<0.01) without altering plasma renin activity.

Structured PICO

Does Angiotensin 1-7 reduce aldosterone secretion in rat models?

Population

Isolated rat glomerulosa cells and rats subjected to a low-salt diet for 7 days

Intervention

Angiotensin 1-7 (in vitro: 10^-6 M; in vivo: continuous infusion 576 μg/kg per day)

Comparator

Vehicle/control

Outcome

Aldosterone secretion/levelssurrogate

Main Result

Absolute Event Rate: 16.4% vs 27.6%

p-value: p=<0.01

Angiotensin 1-7 acts as a negative modulator of aldosterone secretion both in vitro and in vivo, suggesting a counter-regulatory role in the renin-angiotensin-aldosterone system.

Cite This Study

Shefer et al. (2016) studied this question. Angiotensin 1-7 vs. Control/vehicle was evaluated on Aldosterone secretion (p=<0.01). Continuous infusion of Angiotensin 1-7 in rats on a low-salt diet significantly attenuated the rise in aldosterone compared to vehicle (16.4 vs 27.6 ng/dL; P<0.01).

synapsesocial.com/papers/6aa94bc611a60bccbd264ace https://doi.org/10.1161/hypertensionaha.116.07088

Topics

Hypertension management

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Angiotensin 1–7 Is a Negative Modulator of Aldosterone Secretion In Vitro and In Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Topics

Angiotensin 1–7 Is a Negative Modulator of Aldosterone Secretion In Vitro and In Vivo

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Also Consider

Topics

Also Consider