Key result
Pediatric VSD is linked to 10 altered plasma proteins, notably diagnostic markers ALDOB and Tβ4.
Why the study?
Congenital heart disease is one of the largest classes of birth defects, prompting evaluation of differentially abundant plasma proteins in ventricular septal defect.
What plasma proteins are differentially abundant in children with ventricular septal defect compared to healthy children?
Case-Control (n=64)
What plasma proteins are differentially abundant in children with ventricular septal defect compared to healthy children?
p-value: p=<0.05
ALDOB and Tβ4 are identified as potential plasma biomarkers for identifying ventricular septal defect in children.
Should not yet change VSD management; hypothesis-generating for platelet pathways in congenital heart disease.
Congenital heart disease is one of the largest class of birth defects. Eight subjects with ventricular septal defect (VSD, a kind of congenital heart disease) and 11 health children were enrolled in tandem mass tags label-based quantitative proteomic analysis to compare plasma proteins differentially abundance. A total of 66 proteins were significantly upregulated or downregulated in VSD patients compared with healthy children. These proteins were involved in pathways linked to platelet activation, fructose and mannose metabolism, complement and coagulation cascades, glycolysis/gluconeogenesis, regulation of actin cytoskeleton, and carbon metabolism. The amount of ten proteins changed significantly (p < 0.05) in newly recruited 30 VSD compared with 15 control children, which were validated by ELISA. The areas under the receiver operating characteristic curve values of fructose-bisphosphate aldolase B (ALDOB) and thymosin beta-4 (Tβ4) were higher than those of other candidate proteins. ALDOB and Tβ4 might be potential biomarkers applied for identifying VSD in the further works.
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Huang et al. (2019) conducted a case-control in Ventricular septal defect (n=64). Ventricular septal defect vs. Healthy children was evaluated on Differentially abundant plasma proteins (p=<0.05). Ventricular septal defect in children was associated with significant changes in 10 plasma proteins compared to healthy controls (p<0.05), with ALDOB and Tβ4 showing the highest diagnostic value.
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