Key result
Specific hybridization probes enable detection of CVB3 and CVB4 viral RNA in myocardial biopsies.
Why the study?
Hybridization probes specific for CVB3 and CVB4 strains were needed to enable detection of viral RNA sequences in myocardial tissue.
Nucleic acid hybridization probes can be specifically designed to identify CVB3 and CVB4 viral RNA in myocardial tissue, potentially distinguishing virulent from avirulent strains.
Nucleic acid hybridization data are presented showing that hybridization probes can be devised from either empirical data or from comparison of published nucleotide sequences. Hybridization probes specific only for CVB3 strains and CVB4 strains have been devised, which should enable probing of biopsied myocardial tissue for these specific viral RNA sequences. Oligonucleotides based upon published sequence data are presented, which should be able to function as highly sensitive probes, perhaps even distinguishing virulent from avirulent strains of a specific enterovirus.
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Chapman et al. (1987) studied Coxsackie B virus and enterovirus infection. Nucleic acid hybridization probes was evaluated on Development of specific hybridization probes for CVB3 and CVB4 strains. Nucleic acid hybridization probes specific for CVB3 and CVB4 strains were developed to enable probing of biopsied myocardial tissue for viral RNA sequences.
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