An in situ hybridization assay using a coxsackievirus B3 cDNA probe successfully detected enteroviral RNA in the myocardium of infected athymic mice in a disseminated, multifocal manner.
Can an in situ hybridization assay using a coxsackievirus B3 cDNA probe detect enteroviral genomes in myocardial cells?
The development of an in situ hybridization assay provides a potential method for the unequivocal diagnosis of enteroviral heart disease and for studying its pathogenicity.
We have developed an in situ hybridization assay capable of detecting enteroviral RNA in myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group A and B coxsackieviruses and the echoviruses, detection of these various agents commonly implicated in human viral heart disease is possible in a single hybridization assay. We demonstrate the considerable potential of this method for an unequivocal diagnosis of enteroviral heart disease as well as for pathogenicity studies. Using athymic mice persistently infected with coxsackievirus B3 as a model system, we show that the myocardium is affected in a disseminated, multifocal manner.
Kandolf et al. (1987) studied Viral heart disease. In situ hybridization assay using coxsackievirus B3 cDNA probe was evaluated on Detection of enteroviral RNA in myocardial cells. An in situ hybridization assay using a coxsackievirus B3 cDNA probe successfully detected enteroviral RNA in the myocardium of infected athymic mice in a disseminated, multifocal manner.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: