Key result
Titin-BioID mouse model identifies 478 sarcomere-associated proteins, revealing how perinatal signaling and metabolism shape muscle.
Why the study?
Proximity proteomics has not been amenable to study development and disease in vivo, limiting analysis of native protein complexes and subcellular structures in animal models.
The generation of a titin-BioID knock-in mouse enables in vivo proximity proteomics of the sarcomere, revealing developmental shifts in protein networks and supporting the myosin/Z-disc sliding model of contraction.
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Titin-BioID mice enable sarcomere proteomics in vivo; leaves open translation to human muscle development or disease.
Rudolph et al. (2020) studied this question. titin-BioID knock-in vs. Wild-type mice was evaluated on Identification of sarcomeric and sarcomere-associated proteins. The titin-BioID knock-in mouse model identified 478 sarcomere-associated proteins in adult striated muscle, revealing how perinatal signaling and energy metabolism shape muscle cells.
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