Key result
Moderate systemic heparinization improves vessel patency in a new murine interpositional vein grafting model.
Why the study?
A murine model for interpositional vein grafting into an artery is needed to study vein graft stenosis using transgenic and knockout mice.
A new murine model of interpositional vein grafting was successfully developed, demonstrating patency up to 3 months, which can be used for studying vein graft stenosis in transgenic/knockout models.
Provides a platform for genetic studies of vein graft stenosis in mice; should not yet alter clinical microsurgical practice.
A new murine model is presented for interpositional vein grafting into an artery. In ICR (outbred) mice, the femoral vein or a branch of the jugular vein was harvested and placed into a femoral artery defect, using standard microvascular techniques to achieve end-to-end anastomoses with 11-0 nylon suture. The technique requires a high level of microsurgical experience and competence. Success rates (vessel patency) were higher with moderate systemic heparinization (200 units/kg body weight, single i.v. bolus). Vein grafts were successfully transplanted between syngenic C57Bl/6J mice, with patency demonstrated from 1 day to 3 months postoperatively. The model is applicable to studies of vein graft stenosis using murine transgenic/knockout models.
No takes yet. Share an insight, caveat, or question.
Brian C. Cooley (2005) studied Vein grafting (animal model). Interpositional vein grafting into an artery was evaluated on Vessel patency. A new murine model of interpositional vein grafting demonstrated successful vessel patency from 1 day to 3 months postoperatively, with higher success rates using moderate systemic heparinization.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: