Key result
Proteomic profiling links CPA1, GDF-15, and CXCL16 to vascular markers PWV and cIMT independent of ethnicity.
Why the study?
Associations between targeted cardiovascular and inflammatory proteins and phenotypes of deteriorating vascular health, including ethnicity differences, were not well characterized.
Which targeted proteins are associated with deteriorating vascular health phenotypes (hypertension, cIMT, PWV) in African school teachers?
Observational (n=362)
Which targeted proteins are associated with deteriorating vascular health phenotypes (hypertension, cIMT, PWV) in African school teachers?
Multiplex proteomic profiling identified novel associations between specific proteins (such as GDF-15, CXCL16, and CPA1) and markers of deteriorating vascular health, some of which were independent of ethnicity.
Hypothesis-generating for protein markers of vascular phenotypes in African teachers; prospective validation needed before clinical use.
In this observational study, by the use of a multiplex proteomic platform, we aimed to explore associations between 92 targeted proteins involved in cardiovascular disease and/or inflammation, and phenotypes of deteriorating vascular health, with regards to ethnicity.Proteomic profiling (92 proteins) was carried out in 362 participants from the Sympathetic activity and Ambulatory Blood Pressure in Africans (SABPA) study of black and white African school teachers (mean age 44.7 ± 9.9 years, 51.9% women, 44.5% Black Africans, 9.9% with known cardiovascular disease). Three proteins with <15% of samples below detectable limits were excluded from analyses. Associations between multiple proteins and prevalence of hypertension as well as vascular health [Carotid intima-media thickness (cIMT) and pulse wave velocity (PWV)] measures were explored using Bonferroni-corrected regression models.Bonferroni-corrected significant associations between 89 proteins and vascular health markers were further adjusted for clinically relevant co-variates. Hypertension was associated with growth differentiation factor 15 (GDF-15) and C-X-C motif chemokine 16 (CXCL16). cIMT was associated with carboxypeptidase A1 (CPA1), C-C motif chemokine 15 (CCL15), chitinase-3-like protein 1 (CHI3L1), scavenger receptor cysteine-rich type 1 protein M130 (CD163) and osteoprotegerin, whereas PWV was associated with GDF15, E-selectin, CPA1, fatty acid-binding protein 4 (FABP4), CXCL16, carboxypeptidase B (CPB1), and tissue-type plasminogen activator. Upon entering ethnicity into the models, the associations between PWV and CPA1, CPB1, GDF-15, FABP4, CXCL16, and between cIMT and CCL-15, remained significant.Using a multiplex proteomic approach, we linked phenotypes of vascular health with several proteins. Novel associations were found between hypertension, PWV or cIMT and proteins linked to inflammatory response, chemotaxis, coagulation or proteolysis. Further, we could reveal whether the associations were ethnicity-dependent or not.
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Dieden et al. (2021) conducted an observational in Deteriorating vascular health and hypertension (n=362). 92 targeted proteins was evaluated on Prevalence of hypertension and vascular health measures (cIMT and PWV). Proteomic profiling of 92 proteins in 362 participants identified significant ethnicity-independent associations between vascular health markers (PWV, cIMT) and proteins like CPA1, GDF-15, and CXCL16.
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