Key result
Large-scale purification produces completely pure hog kidney renin with a ~4% overall yield.
Why the study?
The purification of renin is challenging due to its extremely low concentration in kidney tissue, necessitating a large-scale purification method.
A 14-step purification procedure successfully isolated 14.6 mg of pure renin from 300 kg of hog kidneys with a 4% yield.
Answers are grounded in this paper and cite their sources.
Pure renin now available at scale for study; extends biochemical tools but leaves clinical translation open.
The complete purification of renin raises difficult problems due to its extremely low concentration in kidney (less than 1/50,000 of total proteins). The complete purification of hog kidney renin has been realized on a large scale, starting from 300 kg of fresh hog kidneys. 14.6 mg of pure renin were obtained with an overall yield of 4%. The purification procedure involved 14 steps. The enzyme was extracted at pH 3.5. Subsequent purification steps were performed in the presence of protease inhibitors to decrease renin proteolysis. These steps included an ammonium sulfate precipitation and a batch-chromatography on DEAE-cellulose. The major purification step was an affinity chromatography on Sepharose-hexamethylene-diaminopepstatin. The enzyme obtained was further purified by molecular sieving gel filtration and isoelectric focusing.
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Sicard et al. (1978) studied this question. Large scale preparation of pure hog kidney renin was evaluated on Yield of pure renin. Complete purification of hog kidney renin was achieved on a large scale from 300 kg of fresh kidneys, yielding of pure renin with an overall yield of 4%.
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