Key result
P2A bicistronic vectors drive higher transgene expression than IRES EMCV in transfected cells.
Why the study?
Multicistronic vectors using IRES EMCV or 2A peptide sequences are used for simultaneous gene expression, but their comparative efficiency is unclear.
Population
Three mammalian cell lines
Comparison
Bicistronic vector with P2A peptide sequence vs bicistronic vector with IRES EMCV
Design
Preclinical study comparing gene expression in transfected cells
Authors
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May guide bicistronic vector choice in gene research; leaves open translation to cardiovascular gene therapy.
Bicistronic vectors utilizing the P2A peptide sequence yield higher transgene expression levels compared to those using IRES EMCV in mammalian cell lines.
Kuzmich et al. (2013) studied this question. Bicistronic vector containing P2A peptide sequence vs. Bicistronic vector based on IRES EMCV was evaluated on Efficiency of gene expression (co-expression levels of RFP and EGFP). Cells transfected with a bicistronic vector containing the P2A peptide sequence exhibited a higher level of transgene expression compared to those with IRES EMCV.