Key result
Overexpression of circ-0010928 inhibited the proliferation, migration, and tube formation of human cardiac microvascular endothelial cells under hypoxic conditions and promoted their apoptosis by acting as a sponge for miR-921.
Circ-0010928 negatively regulates hypoxia-induced angiogenesis in human cardiac microvascular endothelial cells via the miR-921/LSM14A axis, identifying a potential molecular mechanism and therapeutic target for cardiovascular diseases.
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No immediate clinical role for circ-0010928; leaves open functional validation in endothelial angiogenesis.
Background Angiogenesis is an important factor in promoting vascular repair and a valuable process in the treatment of cardiovascular diseases. Circular RNAs (circRNAs) are widely expressed in eukaryotic cells and play an important role in the regulation of endothelial cells (ECs). In our study, bioinformatics analysis and real-time fluorescent PCR detection revealed that circRNA 0010928 (circ-0010928) is differentially expressed in human cardiac microvascular endothelial cells (HCMECs). Material & Methods We evaluated the role of circ-0010928 in HCMECs. Then, we can verify the function of circ-0010928 in HCMECs by cell counting kit-8 (CCK8), scratch test, transwell experiment, tube forming experiment, flow cytometry. Use dual luciferase experiment to detect the binding relationship between circ-0010928, miR-921 and LSM14A. Results Overexpression of circ-0010928 inhibited the proliferation, migration and tube formation of HCMECs under hypoxic conditions and promoted their apoptosis. In addition, dual luciferase reporter assays confirmed that circ-0010928 acted as a sponge of miR-921 and LSM14A as a downstream target gene of miR-921. Silencing miR-921 could also inhibit the proliferation, migration and tube formation of HCMECs and negatively regulate angiogenesis. Conclusion CircRNA-0010928 may inhibit the function of miRNA-921by combining with miRNA-921, and then miRNA-921 plays a role in regulating LSM14A, thereby regulating the state of angiogenesis.
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Zhang et al. (2021) studied Hypoxia-induced angiogenesis. circ-0010928 overexpression vs. Negative control (NC) plasmid was evaluated on Cell proliferation, migration, tube formation, and apoptosis. Overexpression of circ-0010928 inhibited the proliferation, migration, and tube formation of human cardiac microvascular endothelial cells under hypoxic conditions and promoted their apoptosis by acting as a sponge for miR-921.
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