Key result
PDBu drives arterial contraction independently of calcium and myosin light chain phosphorylation.
Why the study?
It was unclear whether phorbol ester-induced contraction of vascular smooth muscle requires calcium-dependent myosin light chain phosphorylation and how its mechanical characteristics compare to calcium-dependent contraction.
Population
Swine carotid artery strips
Comparison
1.0 microM phorbol 12,13-dibutyrate in normal or calcium-free PSS vs calcium-containing 110 mM KCl-PSS
Design
Preclinical experimental study
Authors
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Challenges Ca/MLC-dependent contraction models in arteries; leaves open PKC isoform roles in human vascular disease.
Phorbol ester-induced contraction of swine carotid artery is independent of calcium or MLC phosphorylation, suggesting a role for calcium-independent PKC isoforms in vascular smooth muscle contraction.
Fulginiti et al. (2008) studied this question. Phorbol 12,13-dibutyrate (PDBu) vs. 110 mM KCl-PSS was evaluated on Contraction (stress, stiffness, shortening velocity, MLC phosphorylation). Phorbol 12,13-dibutyrate (PDBu)-induced contraction of the swine carotid artery is not dependent on calcium or myosin light chain phosphorylation.
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