Key result
Endurance training combined with adenosine significantly increased UCP-1 gene expression and decreased MAPK p38 gene expression in the subcutaneous adipose tissue of male rats fed a high-fat diet.
Why the study?
Does endurance training combined with adenosine alter UCP-1 and MAPK p38 gene expression in male rats fed a high-fat diet?
Does endurance training combined with adenosine alter UCP-1 and MAPK p38 gene expression in male rats fed a high-fat diet?
Endurance training combined with adenosine increases UCP-1 and decreases MAPK p38 gene expression in subcutaneous adipose tissue of rats on a high-fat diet, suggesting potential as lipolytic agents in obesity.
Hypothesis-generating for adenosine as exercise adjunct in diet-induced obesity; human translation untested and should not change practice.
Background: Strategies to increase energy expenditure are an attractive approach to reduce excess fat storage and body weight to improve metabolic health. The aim of the present study was to investigate the effects of endurance training combined with adenosine injection on the gene expression of UCP-1 and MAPK p38 in the subcutaneous adipose tissue of male rats fed a high-fat diet. Materials and Methods: Forty rats were randomly divided into 4 groups: 1. normal control, 2. high-fat diet (HFD) control, 3. HFD + adenosine, and 4. HFD + endurance training + adenosine. After 13 weeks of HFD, 12 weeks of endurance training on a moderate-intensity treadmill was performed. UCP-1 and MAPK p38 mRNA levels were measured by RT-PCR. Results: A significant increase in UCP-1 was observed with in HFD + endurance training + adenosine and HFD + adenosine compared to normal and HFD controls. A significant decrease in MAPK p38 was also observed with HFD + endurance training + adenosine and HFD + adenosine compared to HFD. Conclusion: Endurance training and adenosine are likely activators of UCP-1 gene expression and can be used as effective lipolytic agents in obesity. The MAPK p38 pathway increases glucose uptake by insulin and also induces oxidative phosphorylation in mitochondria following a healthy diet and aerobic activity.
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Eslami et al. (2023) studied Obesity (n=40). Endurance training combined with adenosine vs. High-fat diet control was evaluated on Gene expression of UCP-1 and MAPK p38. Endurance training combined with adenosine significantly increased UCP-1 gene expression and decreased MAPK p38 gene expression in the subcutaneous adipose tissue of male rats fed a high-fat diet.
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