Key result
In vitro platelet irradiation to 2000 rads increases lipoxygenase metabolite production by ~23%.
Why the study?
The effect of irradiation on platelet arachidonic acid metabolism and its impact on platelet function had not been specifically determined.
Does irradiation alter arachidonic acid metabolism in platelets in vitro?
Does irradiation alter arachidonic acid metabolism in platelets in vitro?
Absolute Event Rate: 42% vs 34.1%
p-value: p=<0.02
In vitro irradiation of platelets to 2000 rads increases lipoxygenase pathway metabolism without affecting the cyclooxygenase pathway.
In vitro platelet irradiation effects are hypothesis-generating; should not yet inform clinical transfusion practices.
Irradiation of platelets in vitro to 2000 rads did not alter arachidonic acid metabolism through the cyclooxygenase pathway. Production of platelet thromboxane B2 and 12-hydroxy-5,8-10-heptadecatrienoic acid remained similar in control and paired irradiated platelets. In paired samples the platelet lipoxygenase metabolite 12-hydroxy-5,8,10,14-eicosatetraenoic acid was significantly increased postirradiation to 42 +/- 2.8 percent when compared to a control value of 34.1 +/- 3.6 percent (p less than 0.02). Although this latter product has been demonstrated to be chemotactic for neutrophils, its role in platelet function has not been specifically determined.
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Marie J. Stuart (1983) studied Platelet arachidonic acid metabolism. Irradiation vs. Control (unirradiated paired platelets) was evaluated on Platelet lipoxygenase metabolite 12-hydroxy-5,8,10,14-eicosatetraenoic acid production (p=<0.02). In vitro irradiation of platelets to 2000 rads significantly increased the lipoxygenase metabolite 12-hydroxy-5,8,10,14-eicosatetraenoic acid to 42% compared to 34.1% in controls (p<0.02).
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