Key result
In vitro testing underestimates arterial diameter and distensibility by up to ~60% versus in vivo conditions.
Why the study?
Experimental conditions may critically influence the assessment of mechanical properties of carotid arteries, but differences between in vivo, in situ, and in vitro measurements are not well characterized.
How do experimental conditions (in vivo, in situ, in vitro) affect the measured mechanical properties of rat carotid arteries?
How do experimental conditions (in vivo, in situ, in vitro) affect the measured mechanical properties of rat carotid arteries?
Experimental conditions (in vivo vs in vitro/in situ) significantly alter the measured mechanical properties and geometry of conduit vessels like the carotid artery.
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In vitro arterial data may misrepresent in vivo mechanics; leaves open optimal methods for vascular studies in hypertension models.
Zanchi et al. (1998) studied Hypertension. In vitro and in situ conditions vs. In vivo conditions was evaluated on Internal diameter and arterial distensibility. In vitro measurement of rat carotid arteries yielded smaller internal diameters (-33.8% in SHR, -48.3% in WKY) and lower distensibility (-30.1% in SHR, -60.4% in WKY) compared to in vivo conditions.
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