Key result
Baby kidney cell cultures outperform adult kidney cultures for enterovirus isolation and propagation.
Why the study?
Human kidney cultures from different age groups vary in their suitability for isolation and propagation of enteroviruses, but optimal culture conditions were not well established.
Answers are grounded in this paper and cite their sources.
1) Human kidney cultures were prepared successfully from tissues obtained following surgical removal or at autopsy from individuals of all ages, when specimens were collected promptly. 2) Baby kidney cells were superior to adult both for culture growth and for isolation and propagation of enteroviruses. 3) Poliovirus types 1, 2 and 3 (virulent and attenuated strains) grew well in both adult and baby HK cultures. In contrast, Coxsackie A9, B 1-5 and all ECHO viruses tested (except types 7 and 10) induced distinct cellular degeneration only in baby HK cultures, while incomplete destruction with low virus titers were obtained in cultures derived from certain adult human kidneys. 4) The advantages of using baby HK cultures for isolation of certain ECHO viruses are discussed.
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G. D. Hsiung (1959) studied Enterovirus infection. Baby human kidney cultures vs. Adult human kidney cultures was evaluated on Culture growth and isolation/propagation of enteroviruses. Baby kidney cell cultures were superior to adult kidney cultures for the growth, isolation, and propagation of enteroviruses including Coxsackie and ECHO viruses.
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