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September 5, 2025Journal of Agricultural and Food Chemistry

Magnetic Nanoparticle-Assisted Multiplex RPA for Rapid Meat Authentication via Fluorescence Detection

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Authors

CLCuiling LiZWZhen WuDPDaodong Pan

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Overview

This assay demonstrates improved sensitivity for horse and chicken detection in meat, suggesting enhanced authentication methods.

Key Points

  • The assay can detect 0.1% meat adulteration without cross-reactivity, ensuring high specificity.
  • Under optimized conditions, results were achieved in 31.5 minutes for 96 samples with fluorescence detection.
  • mRPA-MS-FD fully aligned with national-standard RT-qPCR in detecting adulterated meat across 102 commercial samples.
  • This platform combines isothermal amplification and magnetic separation for effective meat authentication.

Cite This Study

Li et al. (2025) studied this question.

synapsesocial.com/papers/68bb3a3d2b87ece8dc954f5fhttps://doi.org/10.1021/acs.jafc.5c06794
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