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November 21, 2025FoodsOpen Access

Rapid Detection of Animal-Derived Components in Plant-Based Meat Alternatives Using Recombinase Polymerase Amplification

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Authors

YSYifei SunHLHan LiTMTianQi Ma

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Overview

This work demonstrates rapid detection of animal-derived adulteration in plant-based meat alternatives, highlighting optimization of amplification techniques.

Key Points

  • The study aims to develop a quick and efficient method for detecting animal-derived components in plant-based meats.
  • Evaluated five DNA extraction methods for effectiveness.
  • Designed specific primers for pork, chicken, duck, and beef.
  • Optimized recombinant polymerase amplification conditions for detection.
  • Incorporated SYBR Green I dye for visualization.
  • Achieved detection of adulterants as low as 0.0514% w/w.
  • Significantly reduced detection time and increased sensitivity.
  • Provided a reliable tool for identifying adulteration in plant-based meat alternatives.

Cite This Study

Sun et al. (2025) studied this question.

synapsesocial.com/papers/6924e3f2c0ce034ddc34f049https://doi.org/10.3390/foods14233992
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Also Consider

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  1. 1Rapid detection of murine‐derived ingredients in meat products using real‐time enzymatic recombinase amplification2024
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  4. 4A multiplex DNA probe-based method for simultaneous identification of adulteration in meat samples2024 · 7 citations
  5. 5Establishing a Detection Method Based on Multiplex PCR for Identification of Sheep Meat, Goat Meat and Common Adulterant Meats2025