Figure 6 from Selective Depletion of Cancer Cells with Extrachromosomal DNA via Lentiviral Infection

Integrase-deficient lentivirus does not rescue the effect of integrase-intact lentivirus on ecDNA depletion. A, PC3-NCI and (B) HeLa-MTX-Res cells were either untreated or transduced with Cas9-expressing lentivirus (genome size = 8 kb) generated either using wild-type integrase (Cas9 WT integrase) or point mutant integrase (Cas9 D64V integrase) that does not integrate into the genome. After 48 hours of transduction, cells were cultured for 2 weeks. FISH analysis with oncogene probes was performed to quantify subpopulations (ANOVA; *, P < 0.01; **, P < 0.05; #, P < 0.05; ##, P < 0.01; ns = 2).