Figure 2 from Selective Depletion of Cancer Cells with Extrachromosomal DNA via Lentiviral Infection

Antibiotic treatment does not cause depletion of cells with ecDNA. A, Parental PC3 cells (control), the lentivirally transduced PC3 cells (scrambled), and the PC3 cells that were lentivirally transduced for 48 hours and selected with puromycin for 1 week (scrambled/puromycin) were subjected to FISH analysis with the MYC probe. Cell subpopulations with ecDNA or HSR were quantified based on FISH images (t test, *, P < 0.05; NS = 3). We used the Invitrogen LentiArray CRISPR nontargeting control lentivirus particle for this experiment. B, HeLa-MTX-Res cells (control), the lentivirally transduced HeLa-MTX-Res cells (scrambled), and the HeLa-MTX-Res cells that were lentivirally transduced for 48 hours and selected with puromycin for 1 week (scrambled/puromycin) were subjected to FISH analysis with the DHFR probe. Cell subpopulations with ecDNA or HSR were quantified based on FISH images (t test, *, P < 0.05; NS = 3). We used the Invitrogen LentiArray CRISPR nontargeting control lentivirus particle for this experiment.