Figure 3 from Selective Depletion of Cancer Cells with Extrachromosomal DNA via Lentiviral Infection

Alternative ways to deliver transgenes to cells without population shift. A, Larger transgene/plasmid size in lentiviral constructs depletes ecDNA+ cells with the expansion of HSR+ cells. PC3 cells were either untreated or lentivirally transduced with the indicated plasmids for 48 hours and selected with blasticidin for 2 weeks. FISH analysis with the MYC probe was performed to quantify subpopulations (ANOVA, *, P < 0.0001; n = 3). B, Non-lentiviral (PiggyBac) plasmid does not deplete ecDNA+ cells. PC3 cells were either untreated or lentivirally transduced with the Cas9 plasmid (Lenti-Cas9) or transfected with the PiggyBac-Cas9 plasmid for 48 hours and selected with blasticidin for 2 weeks. FISH analysis with the MYC probe was performed to quantify subpopulations (ANOVA, *, P < 0.0001; n = 3).