Figure 4 from Selective Depletion of Cancer Cells with Extrachromosomal DNA via Lentiviral Infection

ecDNA reintegration does not cause the depletion of the ecDNA+ population. A, Relative quantification of MYC copy was tested by qPCR in parental, ecDNA+, HSR+, and lentivirally transduced PC3 cell lines (t test, **, P < 0.01; ns = 4). B, Copy-number variation (CNV) analysis results show a comparable copy-number ratio (CR) around the MYC amplification region between parental and transduced PC3 cells. C, CNV analysis results show a comparable CR around the DHFR amplification region between parental and transduced HeLa-MTX-Res cells. D, AmpliconArchitect results show a high similarity in the MYC amplicon structure between parental and transduced PC3 cells. *The closer it is to 1 in the range of 0–1, the more similar the structure is. E, AmpliconArchitect results show a high similarity in the DHFR amplicon structure between parental and transduced HeLa-MTX-Res cells. *The closer it is to 1 in the range of 0–1, the more similar the structure is.