Figure 4 from Inhibition of Extracellular Matrix Protein Fibulin-3 Reduces Immunosuppressive Signaling and Increases Macrophage Activation in Glioblastoma

Fibulin-3 regulates the expression of immunomodulatory signals in GBM cells via NF-κB signaling. A, Expression of CSF-1 mRNA in U251MG cells cotransfected with fibulin-3 cDNA and p65/RelA siRNA, or their respective controls. B–D, Expression of CSF-1 (B), CD47 (C), and TGF-β1 (D) in control and fibulin-3–overexpressing U251MG cells in presence of the NF-κB inhibitor, CAPE (20 μmol/L), or its vehicle. E, Expression of CSF-1 mRNA in GBM9 GSCs transfected with control or p65/RelA siRNAs and exposed overnight to purified fibulin-3 (500 ng/mL). F–H, Expression of CSF-1 (F), CD47 (G), and TGF-β1 (H) in GBM9 cells exposed to purified fibulin-3, CAPE, or their respective vehicles. The results in all panels were analyzed by two-way ANOVA.